Principles and Technical Aspects of PCR Amplification by: Elizabeth van Pelt-Verkuil, Alexander van Belkum, John P. Hays Publisher: Springer 04-2008 ISBN: 1402062400 339 pages 20 Mb
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than 15 years ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical, diagnostic and academic laboratories across the world. Further, these techniques are being applied at the diagnostic level (e.g. as high throughput testing methodologies to detect minimum residual disease, the presence/absence of specific pathogens etc), as well as to increase our understanding of fundamental disease processes.